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03 - 06 July 2017, Manchester, UK

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Cryo-Correlative Microscopy – Improving the work flow

Linkam has been developing cryo stages for correlative microscopy for many years and continues to be at the forefront of Cryo-Correlative microscopy with the latest update of their LINK software for the CMS196M providing improved imaging capabilities. Also added is the new liquid nitrogen LN2 Autofill system providing longer run times. Learn more about Cryo-Correlative microscopy at mmc2017. See us at stand number 208.

Cryo-Correlative Microscopy has become an established technique in recent years bringing together the strengths of both Electron and Light (fluorescence) Microscopy. Electron microscopy (EM) provides structural information at very high resolution. However, it can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes. Fluorescence microscopy on the other hand is an extremely sensitive technique to detect biological, chemical and genetic processes and events inside living cells.

Cryo-CLEM brings it all together: combining the individual advantages from both fluorescence and EM by imaging the same sample location with both techniques and superimposing the complementary information.

For biological samples to be compatible with the vacuum conditions found in EM and preserve the structural detail, samples are embedded in vitrified “glass like” ice and need to be kept below -140 °C. Any contact with moisture contained in the air has to be avoided since ice crystals would form immediately and contaminate the sample. Under cryo-conditions the structural detail is preserved and photo bleaching is significantly reduced.

The CMS196M provides a complete solution for fluorescence imaging of cryo prepared EM specimens. The integrated high resolution, encoded XY scanning mechanism and LINK provides complete control and monitoring of the system as well as fully automated tiled image capture providing the ability to capture an image of the entire EM grid at full resolution – see below. The system produces a single image of the EM grid that can then be used to navigate the sample and save co-ordinates of areas of interest making it easy to navigate to the same area when the sample is transferred to the EM.

mmc2017 is great opportunity to find out more about Cryo-CLEM. On Wednesday 5th July, there is a session focussed on correlative techniques and Linkam will be running a workshop during the lunch break to show the CMS196M as well as to introduce the next generation of plunge freezers. It’s the ideal time to come and talk to us about your needs and how Linkam systems can fit right into your Cryo-Correlative workflow.

If you are not able to come to see us in Manchester, please do visit our web site and learn more about our approach to CLEM and other materials characterisation challenges:

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mmc2017 is the latest in an impressive list of events dating back to the 1960s starting with the Micro conference and exhibition series. This later became MicroScience which grew ever-bigger until 2010. The RMS hosted the European Microscopy Congress in 2012 which was the catalyst for the current Microscience Microscopy Congress series.

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