Fabrication, Characterization and Studies on DPPH Scavenging Activity of Silver Nanoparticles by Using Rumex hastatus

Session

Poster Session One

Authors

Uzma Bashir (1), Hafiz Muhammad Asif (1), Rabia Zahid (1), Abdul Hamid khan (2), Misbah Firdous (1), Ahsan Zahid (1), Muhammad Hasnain (1)

Affiliations

1. The Islamia University of Bahawalpur
2. university of poonch rawalakot

Keywords

Keywords: Rumex hastatus, Nanoparticles, Metals, Antioxidant, Hypertension

Abstract text

Among metal nanoparticles, silver nanoparticles (Ag-NPs) explicitly are recognized for their acceptable presentations in therapeutic. Silver has been glorious, for quite 2000 years, as a metal that shows sensible medical effects, compounds containing silver being employed in different biological functions. Current study aimed to develop non-toxic, eco-friendly and cost-effective approaches for silver nanoparticle (Ag-NPs) synthesis using whole medicinal plant of Rumex hastatus,  characterization using various analytical techniques and free radical scavenging activity of extract and silver nanoparticles that is used as traditional medicinal practice for hypertension. Besides, the crystallinity of Ag+, reduction/stability sample gave λmax peaks in between 400-500nm (UV-vis spectra). Fourier transforms infrared spectroscopy (FT-IR spectroscopy) shown functional groups such as unsaturated ketone, alcohol, aldehyde and structural morphology were determined through X-ray diffraction (XRD) which shown strong reflection at 39.50° to determine the formation of nanocrystals.  Some particles seem massive, others have apparent sizes as tiny as 10µm and 0.5 µm in scanning electron microscopy (SEM) respectively. Hydro-ethanolic extract prepared with the percentage yield of 8%.  The hydro-ethanolic plant extract (sample 1) and nano particle (sample 2) shown high radical scavenging activity. This study showed that IC50 DPPH radical inhibition of sample 1 and 2 was 58.59 µg/ml, 18.22µg/ml. The percentage inhibition of sample 1 was 74.92 ±1.0, 61.89 ±1.00, 48.32 ±1.04 at the concentration of 250, 125, 62.5µg/ml and sample 2 was 83.67 ±1.00, 69.87 ±1.00, 55.82 ±1.00 and 41.8 ±1.00 at the concentration of 100, 50, 25, 12.5µg/ml.  IC50 shown that sample 2 was more strong active then sample I as lower IC50 reflect higher radical scavenging activity. The value of p was <0.05 which shown the significance of antioxidant activity. In future further studies are needed to evaluate the effects of silver nanoparticles of Rumex hastatus in cardiovascular diseases specially hypertension in vivo studies.