The Oxford Particle Imaging Centre (OPIC), UK

Session

Cryo-Electron Microscopy

Authors

Dr Helen Duyvesteyn (1), Dr. Loic Carrique (1), Professor Jon Grimes (1, 2)

Affiliations

1. Oxford Particle Imaging Centre, the Division of Structural Biology, University of Oxford
2. Diamond Light Source

Keywords

TEM, SEM, Aquilos2, Krios, Glacios, Cellular tomography, EM Facility, Cryo-electron microscopy, FIB-SEM, Falcon-4, Falcon-4i, SelectrisX, Arctis, PFIB-SEM, single particle, tomography, life sciences, virology, high-containment, Instruct

Abstract text

The OPIC EM facility, an Instruct centre at the University of Oxford, offers a wealth of EM resources and expertise to academia and industry-based applicants for single-particle and tomography EM experiments. Over the past couple of years, we have undergone significant upgrades, including the installation and commissioning of an Aquilos2+ FIB/SEM, upgrades of our Glacios screening microscope and Krios G3i microscope with a Falcon-4/SelectrisX detector and energy filter. We are now eagerly waiting for our Arctis plasma-FIB/SEM, scheduled to arrive later this year, in addition to a Falcon4i detector. Working in synergy with eBIC (Harwell, UK), we have the unique capability of preparing and collecting TEM data on Biosafety-level 3 (BSL3) virus and cellular samples using our high-end equipment housed in a BSL3 containment suite.

Access to our facility is available at request from academia and industry alike for competitive prices, and funding is available via Instruct.