Deconstructing the tumour microenvironment in glioblastoma using imaging mass cytometry

Abstract number
304
DOI
10.22443/rms.mmc2023.304
Corresponding Email
[email protected]
Session
Spatial and Imaging Cytometry
Authors
Dr Michael Haley (1), Leoma Bere (1), Prof Kevin Couper (1)
Affiliations
1. University of Manchester
Keywords

glioblastoma, tumour microenvironment, imaging mass cytometry, high dimensional imaging

Abstract text

Glioblastoma (GBM) treatment lags behind other cancers primarily because of its cellular and spatial heterogeneity. We do not understand how different neoplastic and immune cell subsets interact, or where their interactions occur in the tumour, or how these interactions affect GBM disease trajectory. Imaging mass cytometry (IMC) is a new technology that allows imaging of 40+ protein markers in tissues, giving new insights into how tissues are spatially organised at the cellular level, and how cells interact in health and disease. We have therefore used IMC in combination with novel bioinformatics approaches to deconstruct the tumour microenvironment and understand how neoplastic and immune cell interact in glioblastoma, and how these interactions may affect disease outcome.