Spectroscopic Coherent Raman Imaging as a Discovery Tool for Biology

Session

Advances in Label-free Imaging

Authors

Professor Marcus Cicerone (1)

Affiliations

1. Georgia Institute of Technology

Keywords

Coherent Raman Imaging, Spectroscopic Imaging

Abstract text

Spectroscopic coherent Raman imaging (CRI) methods allow label-free, chemically specific imaging of materials and biological systems and open many exciting possibilities for understanding phenomena in these systems. I will introduce spectroscopic CRI, focusing on broadband coherent anti-Stokes Raman scattering (BCARS) microscopy. I will discuss approaches we have adopted to optimize signal generation with a limited photon budget and approaches we use to extract information of interest from our high-dimensional image data. I will also report on our use of BCARS to identify and explore a previously unknown biogenesis pathway in viral replication and our early efforts to develop it as a situational metabolomics surrogate.

References

 Chen, W.-W. et al. Spectroscopic coherent Raman imaging of Caenorhabditis elegans reveals lipid particle diversity. Nature Chemical Biology 3, 1–9 (2020).  

Camp, C. H. et al. High-speed coherent Raman fingerprint imaging of biological tissues. Nature photonics 8, 627–634 (2014).