The power of SIM as a tool for biological discovery
- Abstract number
- 315
- Corresponding Email
- [email protected]
- Session
- Multimodal Microscopy
- Authors
- Prof Lothar Schermelleh (1)
- Affiliations
-
1. University of Oxford
- Keywords
Super-resolution microscopy, structured illumination, chromatin organisation, cohesin
- Abstract text
Super-resolution microscopy has undergone a remarkable evolution over the past two decades. However, despite its promise, it has not always satisfied expectations when it comes to widespread adoption in biomedical research labs. In my talk, I will point out the unique properties of interference-based structured illumination microscopy (SIM) for addressing biological questions as a tool for discovery and present our efforts to make SIM more reproducibly applicable. I will further demonstrate the power of SIM for quantitative 3D super-resolution imaging by showcasing our recent work on exploring mammalian chromatin topography and investigating cell-cycle dependent cohesion complex organisation. I will also highlight some of the technical advances in fluorescence labelling and image analyses that were required along the way.